INTRODUCTION: Positive and negative Kaposi sarcoma-associated (KSA) individuals need an improved diagnostic assay for human herpesvirus-8 (HHV-8) detection. The selection of one or more viral epitopes that elicit strong immune responses is essential in developing this assay.
INTRODUCTION: Human herpesvirus-8 (HHV-8) is a γ-herpesvirus which is related to the Epstein-Barr virus and is etiologically associated with Kaposi’s sarcoma (KS). The AIDS epidemic over the last 40 years has resulted in a high rate of KS due to immunosuppression. The seroprevalence for HHV-8 varies from 1-5% in the US to up to 80% […]
INTRODUCTION: Human Herpes Simplex Type 2 (HSV-2) causes a life-long infection and occurs in over 10% of adult individuals. This report details the approach by Goux et al. (2019) to identify HSV-2 infections using a Smartphone to detect the nanophosphor signal of a lateral flow device. DISCUSSION: Lateral flow assays (LFAs) are needed to improve […]
INTRODUCTION: The detection of a single analyte in a lateral flow assay or lateral flow device (LFD) is a common, low cost, rapid diagnostic assay. There is, however, a need to detect more than one analyte in a single assay to further reduce the costs and time involved in the detection of multiple analytes. A […]
INTRODUCTION: Lateral flow assays (LFA) have been used for several decades to detect chemicals, biologically relevant proteins, toxins, metals, and specific disease-related analytes (either bacterial or viral). The low cost and ease of use make these assays ideal for point-of-care diagnostics. One of the disadvantages of LFAs has been that they are prone to false […]
INTRODUCTION: Lateral flow assays (LFAs) are a widely used diagnostic tool for point-of-care diagnostics. LFAs require optimization of the component assembly, sample pad, conjugate pad, nitrocellulose membrane (NCM), and absorbent pad on the plastic backing in preparation for a specific assay. DISCUSSION: Components to be optimized include analytical membranes (typically NCM), conjugate and sample pads, […]
LAMINATION/CUTTING OF LFA STRIPS: LM5000 The LM5000 Clamshell assembles a lateral flow assay comprised of multiple materials onto an adhesive backing. It contains top and bottom vacuum nests to hold strip materials in place and is operated manually. Standard and customized nest inserts are available and are easily interchangeable so that multiple assay designs can […]
DISPENSE PLATFORMS, continued: The XYZ series systems are a powerful production tool for rapid test development and manufacturing with a larger working deck than the smaller XYZ3060 platform. XYZ3210 The XYZ3210 is a flexible XYZ motion/dispense system, with a motored Z-axis and fully programmable motion and dispense parameters. The XYZ3210 is designed for simultaneous dispensing […]
BioDot provides a number of reagent dispensing platforms that are designed for a combination of R&D and production activities. In both cases, the same dispensing technology is used which allows for R&D validated processes to be efficiently transferred to the manufacturing environment. IMPREGNATION: Bulk impregnation of sheets in baths or in-line with the RR120 is […]
DISPENSING AND IMPREGNATION PRODUCTS, continued: AirJet HR™ The AirJet HR™ is a nanoliter aerosol dispenser. This BioDot system uses pressurized air to atomize fluid passing through the dispensing nozzle for non-contact, quantitative aerosol dispensing. This creates a quantitative spray format, in which a dot or line can be quantitatively generated on a continuous basis. BioDot’s […]