Entries by Viro Research

Manufacturing of Lateral Flow Assays (Part 4)

BioDot provides a number of reagent dispensing platforms that are designed for a combination of R&D and production activities. In both cases, the same dispensing technology is used which allows for R&D validated processes to be efficiently transferred to the manufacturing environment. IMPREGNATION: Bulk impregnation of sheets in baths or in-line with the RR120 is […]

Manufacturing of Lateral Flow Assays (Part 3)

DISPENSING AND IMPREGNATION PRODUCTS, continued: AirJet HR™ The AirJet HR™ is a nanoliter aerosol dispenser. This BioDot system uses pressurized air to atomize fluid passing through the dispensing nozzle for non-contact, quantitative aerosol dispensing. This creates a quantitative spray format, in which a dot or line can be quantitatively generated on a continuous basis. BioDot’s […]

Manufacturing of Lateral Flow Assays (Part 2)

DISPENSING AND IMPREGNATION PRODUCTS, continued: The next several systems are non-contact, which is the preferred system with LFA diagnostic assay applications. All of these are offered by BioDot. BioJet HR™ The BioJet HR™ is a proprietary, qualitative, non-contact technology which couples the BioJet “Drop-on-Demand” valve with a high-resolution syringe pump. The BioJet HR™ meters precise […]

Manufacturing of Lateral Flow Assays (Part 1)

INTRODUCTION: There are many companies who provide equipment for making diagnostic LFAs. The LFA device is a test strip inside a plastic housing. This housing can have two or more ports for sample analysis. The strips have five components which include: 1) plastic backing with adhesive and release liner, 2) sample pad, 3) conjugate pad, […]

Multiplexing the Lateral Flow Immunoassay (LFIA)

INTRODUCTION: Relatively few multiplex lateral flow immunoassays (LFIAs) are available today for detecting viral pathogens, primarily due to technical and manufacturing hurdles. However, several of these assays are being developed. The detection of antibodies against more than one agent is probably the easiest type of multiplex assay to manufacture. The key components of such assays […]

Lateral Flow Assays: Advantages-Disadvantages

INTRODUCTION: Lateral flow immunoassays (LFIAs) are an important component in point-of-care patient diagnostics. More LFIAs are being developed every year, driven by the need of rapid, low-cost information in a patient or hospital setting. Presented in this note will be the overall advantages and disadvantages to LFIAs, as well as new research to improve the […]

Lateral Flow Assay (LFA) Reviews

INTRODUCTION: In the last several years there has been an increasing demand for the development of infectious disease diagnostics which maintains test accuracy while reducing the time needed for detection at the point-of-care (POC) for the patient. Reducing the cost per assay as well as the equipment and end-user technical knowledge has been the driving […]

Expression of HSV-1 and -2 Antigens for Diagnostic Assays

By David Kilpatrick, PhD and Abbas Vafai, PhD INTRODUCTION: The detection and differentiation of HSV-1 & -2 infections present challenges in a hospital point of care environment. There are many laboratory tests which can be performed, including serological assays, polymerase chain reactions, as well as virus neutralization and/or culture. However, only simple, quick serological assays, […]

Comparison of three HSV-2 immunoassays: Focus-Kalon-BioKit

By David Kilpatrick, PhD and Abbas Vafai, PhD INTRODUCTION: Various assays have been developed over the last thirty years for detecting Herpes virus antibodies in patient sera. The three most commonly used assays are Western blot assay (WBA), enzyme-linked immunosorbent assay (ELISA), and enzyme immnodot assays (EIA). Of these, the Western blot assay (WBA) is […]

The need for a more reliable commercial test for HSV

By David Kilpatrick, PhD and Abbas Vafai, PhD INTRODUCTION: There are several commercial immunoassays for HSV on the market (Western blot, Dot blot or standard ELISA assays). Of these, the University of Washington Western blot assay (UW-WB) is considered the “gold standard” for assays to detect Herpes virus antibodies in patient sera. While this FDA […]