Entries by Viro Research

Inventor of SHINGRIX Develops New Chickenpox and Shingles Virus Detection Test

Abbas Vafai, Ph.D. inventor of shingles vaccine, SHINGRIX, develops ZosterGent® VZV Identification Reagent, a more sensitive test in detecting the shingles virus. SNELLVILLE, Ga., June 9, 2020 (Newswire.com) – Viro Research, developer of high quality biologics and reagents, announced today the launch of ZosterGent® for lesion testing of at-risk patients suspected of having chickenpox or […]

A Lateral Flow Assay for Detecting IgG/IgM for COVID-19

INTRODUCTION: Numerous labs are developing antibody assays to detect COVID-19. There are presently (as of May 1, 2020) four FDA approved assays for detecting IgG/IgM and three for detecting IgG only. Four of these assays use a lateral flow assay (LFA) architecture. All of the assays use the viral S1 glycoprotein as the antibody target. […]

Detection of HHV-8 Associated with Kaposi’s Sarcoma

INTRODUCTION: Human herpesvirus-8 (HHV-8) is a γ-herpesvirus which is related to the Epstein-Barr virus and is etiologically associated with Kaposi’s sarcoma (KS). The AIDS epidemic over the last 40 years has resulted in a high rate of KS due to immunosuppression. The seroprevalence for HHV-8 varies from 1-5% in the US to up to 80% […]

Nanophosphor Lateral Flow Assay for Detecting HSV-2

INTRODUCTION: Human Herpes Simplex Type 2 (HSV-2) causes a life-long infection and occurs in over 10% of adult individuals. This report details the approach by Goux et al. (2019) to identify HSV-2  infections using a Smartphone to detect the nanophosphor signal of a lateral flow device. DISCUSSION: Lateral flow assays (LFAs) are needed to improve […]

Three Color, Multiplex LFA

INTRODUCTION: Lateral flow assays (LFA) have been used for several decades to detect chemicals, biologically relevant proteins, toxins, metals, and specific disease-related analytes (either bacterial or viral). The low cost and ease of use make these assays ideal for point-of-care diagnostics. One of the disadvantages of LFAs has been that they are prone to false […]

Optimization of Lateral Flow Assays

INTRODUCTION: Lateral flow assays (LFAs) are a widely used diagnostic tool for point-of-care diagnostics. LFAs require optimization of the component assembly, sample pad, conjugate pad, nitrocellulose membrane (NCM), and absorbent pad on the plastic backing in preparation for a specific assay. DISCUSSION: Components to be optimized include analytical membranes (typically NCM), conjugate and sample pads, […]

Manufacturing of Lateral Flow Assays (Part 6)

LAMINATION/CUTTING OF LFA STRIPS: LM5000 The LM5000 Clamshell assembles a lateral flow assay comprised of multiple materials onto an adhesive backing. It contains top and bottom vacuum nests to hold strip materials in place and is operated manually. Standard and customized nest inserts are available and are easily interchangeable so that multiple assay designs can […]

Manufacturing of Lateral Flow Assays (Part 5)

DISPENSE PLATFORMS, continued: The XYZ series systems are a powerful production tool for rapid test development and manufacturing with a larger working deck than the smaller XYZ3060 platform. XYZ3210 The XYZ3210 is a flexible XYZ motion/dispense system, with a motored Z-axis and fully programmable motion and dispense parameters. The XYZ3210 is designed for simultaneous dispensing […]