Learn more on the topic of Varicella Zoster Virus (VZV) via our ongoing series of clinical posts by David Kilpatrick, PhD and Abbas Vafai, PhD.
By David Kilpatrick, PhD and Abbas Vafai, PhD INTRODUCTION: Various assays have been developed over the last thirty years for detecting Herpes virus antibodies in patient sera. The three most commonly used assays are Western blot assay (WBA), enzyme-linked immunosorbent assay (ELISA), and enzyme immnodot assays (EIA). Of these, the Western blot assay (WBA) is […]
By David Kilpatrick, PhD and Abbas Vafai, PhD INTRODUCTION: There are several commercial immunoassays for HSV on the market (Western blot, Dot blot or standard ELISA assays). Of these, the University of Washington Western blot assay (UW-WB) is considered the “gold standard” for assays to detect Herpes virus antibodies in patient sera. While this FDA […]
By David Kilpatrick, PhD and Abbas Vafai, PhD INTRODUCTION: Finding antigens which can be used for human Herpes virus (HHV) assays can be challenging. Many technical concerns need to be addressed, including how to express sufficient quantities of each antigen, and once produced, how the immune system responds to each antigen. In this paper, Brenner […]